What invalidating breast cancer gene patents implies
Another embodiment of the present invention is the wild-type by at least one mutation in conjunction with any of the proteins, particularly wild-type amino acid sequence of any nucleic acid molecule, or the above-described mutant Ras proteins of the above-described nucleic acid molecule 76 in position K -ras, N-ras or H-ras amino acid sequence and is let to selectively catalyze the inactivation of the ras gene encoding a mutated Ras protein containing the amino acid sequence distinct from Lai relates to Im.본 발명의 또 다른 구체예는 상기 기술된 핵산 분자 중 임의의 핵산 분자, 또는 상기 기술된 돌연변이 Ras 단백질 중 임의의 단백질, 특히 야생형 아미노산 서열과 관련하여 적어도 76번 위치의 돌연변이에 의해 야생형 K-ras, N-ras 또는 H-ras 아미노산 서열과는 구별되는 아미노산 서열을 포함하는 돌연변이 Ras 단백질을 코딩하는 ras 유전자에 매우 높은 엄격 조건(very high stringency conditions) 하에서 혼성화하고 상기 핵산 분자 또는 유전자의 발현을 저해하는 안티센스 핵산 분자에 관한 것이다.
Another embodiment of the present invention is of a medicament for preventing or treating cancer of said any of the aptamer, the techniques described any si RNA, let Lai any of the above techniques of, or the above-described random antisense nucleic acid molecule It relates to the use for the manufacture.Amino acid is found in the Ras mutation also various cancers in 13, 59 and 61 (e. 5084-8; Abrams et al, Sem Oncol 1996 23, 118 -134; see Friday and Adjei, Biochim Biophys Acta 2005 1756, 127-144).Malignant cell growth or cancer is a worldwide leading cause of death, the development of effective therapies for neoplastic disease is a subject of enormous research.Another embodiment of the present invention have a cancer, or in an animal at risk of cancer occurs, the wild-type amino acid sequence and related to the wild-type K-ras, N-ras or H-ras amino acid by mutation of at least 76-position sequence and It is directed to a method for preventing or treating cancer which comprises administering a compound that inhibits the expression of the mutated Ras protein containing the amino acid sequence distinct.본 발명의 또다른 구체예는 암을 가지고 있거나 암이 발생할 위험이 있는 동물에서, 야생형 아미노산 서열과 관련하여 적어도 76번 위치의 돌연변이에 의해 야생형 K-ras, N-ras 또는 H-ras 아미노산 서열과는 구별되는 아미노산 서열을 포함하는 돌연변이 Ras 단백질의 GTP 가수분해를 개시 또는 촉발시키는 화합물을 투여하는 것을 포함하는 암의 예방 또는 치료 방법에 관한 것이다.Another embodiment of the present invention have a cancer, or in an animal at risk of cancer occurs, the wild-type amino acid sequence and related to the wild-type K-ras, N-ras or H-ras amino acid by mutation of at least 76-position sequence and It is directed to a method for preventing or treating cancer which comprises administering a compound to initiate or trigger the GTP hydrolysis of the mutant Ras proteins comprising an amino acid sequence that is distinct.In another embodiment, the present invention is a set of different nucleic acid molecules, i.e., 59 times, 61 times, 73 times, 74 times, 75 times, 76 times, at least one mutation at a position selected from 77 or 78-position one or more containing at least one additional mutation but are not more than one nucleic acid molecule and thereby limit containing (preferably from one to 78 times 73 locations selected from any one of a), including the G12 mutant, and G13 mutant additional nucleic acid It provides a combination of molecules.
Another embodiment of the invention relates to a recombinant nucleic acid molecule comprising any of the nucleic acid molecule of the above-described nucleic acid molecules in one or more expression control sequence operably linked.
In one aspect, the detecting step is the polymerase chain reaction (PCR), reverse transcriptase from -PCR (RT-PCR), in situ hybridization, Northern blot, sequence analysis, the group consisting of gene microarray analysis and detection of reporter gene It is caused by the selected method.
In one aspect, the nucleic acid sequence level of a nucleic acid sequence encoding the mutated Ras protein is brought into contact with the nucleic acid isolated from the selective primer or probe and the test sample which binds to the nucleic acid sequence encoding the mutated Ras protein, encoding the mutated Ras protein It is determined by detecting whether the coupling by which the primer or probe.
Ras mutations in the codon encoding the amino acid 12 of the protein was 78% and cancer, 34% of colon carcinoma and 40% of the ratio of the - is found in ovarian cancer small cell lung carcinoma and 24%.아미노산 13, 59 및 61에서의 Ras 돌연변이 또한 다양한 암에서 발견된다(예컨대 , Lu et al., Cancer Res.
2004 Aug 1;64(15):5084-8; Abrams et al, Sem Oncol 1996 23, 118-134; Friday 및 Adjei, Biochim Biophys Acta 2005 1756, 127-144 참조).
Despite proposed a variety of innovative methods for the prevention and treatment of cancer, many cancer they can still cause high mortality rates, relatively difficult to treat, or not respond to traditional therapies.