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Amidating enzyme

It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins. This subsection of the PTM / Processing section describes a propeptide, which is a part of a protein that is cleaved during maturation or activation. Cyclic redundancy and other checksums Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)) 10 20 30 40 50MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV 60 70 80 90 100PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT 110 120 130 140 150VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG 160 170 180 190 200FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY 210 220 230 240 250LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV 260 270 280 290 300RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH 310 320 330 340 350IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP 360 370 380 390 400VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQDFH MEEALDWPGV 410 420 430 440 450YLLPGQVSGV ALDPKNNLVI FHRGDHVWDG NSFDSKFVYQ QIGLGPIEED 460 470 480 490 500TILVIDPNNA AVLQSSGKNL FYLPHGLSID KDGNYWVTDV ALHQVFKLDP 510 520 530 540 550NNKEGPVLIL GRSMQPGSDQ NHFCQPTDVA VDPGTGAIYV SDGYCNSRIV 560 570 580 590 600QFSPSGKFIT QWGEESSGSS PLPGQFTVPH SLALVPLLGQ LCVADRENGR 610 620 630 640 650IQCFKTDTKE FVREIKHSSF GRNVFAISYI PGLLFAVNGK PHFGDQEPVQ 660 670 680 690 700GFVMNFSNGE IIDIFKPVRK HFDMPHDIVA SEDGTVYIGD AHTNTVWKFT 710 720 730 740 750LTEKLEHRSV KKAGIEVQEI KEAEAVVETK MENKPTSSEL QKMQEKQKLI 760 770 780 790 800KEPGSGVPVV LITTLLVIPV VVLLAIAIFI RWKKSRAFGD SEHKLETSSG 810 820 830 840 850RVLGRFRGKG SGGLNLGNFF ASRKGYSRKG FDRLSTEGSD QEKEDDGSES 860 EEEYSAPLPA LAPSSS 10 20 30 40 50MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV 60 70 80 90 100PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT 110 120 130 140 150VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG 160 170 180 190 200FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY 210 220 230 240 250LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV 260 270 280 290 300RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH 310 320 330 340 350IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP 360 370 380 390 400VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQGDF YSLLSKLLGE 410 420 430 440 450REDVVHVHKY NPTEKAESES DLVAEIANVV QKKDLGRSDA REGAEHERGN 460 470 480 490 500AILVRDRIHK FHRLVSTLRP PESRVFSLQQ PPPGEGTWEP EHTGDFHMEE 510 520 530 540 550ALDWPGVYLL PGQVSGVALD PKNNLVIFHR GDHVWDGNSF DSKFVYQQIG 560 570 580 590 600LGPIEEDTIL VIDPNNAAVL QSSGKNLFYL PHGLSIDKDG NYWVTDVALH 610 620 630 640 650QVFKLDPNNK EGPVLILGRS MQPGSDQNHF CQPTDVAVDP GTGAIYVSDG 660 670 680 690 700YCNSRIVQFS PSGKFITQWG EESSGSSPLP GQFTVPHSLA LVPLLGQLCV 710 720 730 740 750ADRENGRIQC FKTDTKEFVR EIKHSSFGRN VFAISYIPGL LFAVNGKPHF 760 770 780 790 800GDQEPVQGFV MNFSNGEIID IFKPVRKHFD MPHDIVASED GTVYIGDAHT 810 820 830 840 850NTVWKFTLTE KLEHRSVKKA GIEVQEIKDS EHKLETSSGR VLGRFRGKGS 860 870 880 890 900GGLNLGNFFA SRKGYSRKGF DRLSTEGSDQ EKEDDGSESE EEYSAPLPAL APSSS 10 20 30 40 50MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV 60 70 80 90 100PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT 110 120 130 140 150VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG 160 170 180 190 200FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY 210 220 230 240 250LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV 260 270 280 290 300RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH 310 320 330 340 350IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP 360 370 380 390 400VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQGDF YSLLSKLLGE 410 420 430 440 450REDVVHVHKY NPTEKAESES DLVAEIANVV QKKDLGRSDA REGAEHERGN 460 470 480 490 500AILVRDRIHK FHRLVSTLRP PESRVFSLQQ PPPGEGTWEP EHTGDFHMEE 510 520 530 540 550ALDWPGVYLL PGQVSGVALD PKNNLVIFHR GDHVWDGNSF DSKFVYQQIG 560 570 580 590 600LGPIEEDTIL VIDPNNAAVL QSSGKNLFYL PHGLSIDKDG NYWVTDVALH 610 620 630 640 650QVFKLDPNNK EGPVLILGRS MQPGSDQNHF CQPTDVAVDP GTGAIYVSDG 660 670 680 690 700YCNSRIVQFS PSGKFITQWG EESSGSSPLP GQFTVPHSLA LVPLLGQLCV 710 720 730 740 750ADRENGRIQC FKTDTKEFVR EIKHSSFGRN VFAISYIPGL LFAVNGKPHF 760 770 780 790 800GDQEPVQGFV MNFSNGEIID IFKPVRKHFD MPHDIVASED GTVYIGDAHT 810 820 830 840 850NTVWKFTLTE KLEHRSVKKA GIEVQEIKGK GSGGLNLGNF FASRKGYSRK 860 870 880 GFDRLSTEGS DQEKEDDGSE SEEEYSAPLP ALAPSSS 10 20 30 40 50MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV 60 70 80 90 100PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT 110 120 130 140 150VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG 160 170 180 190 200FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY 210 220 230 240 250LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV 260 270 280 290 300RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH 310 320 330 340 350IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP 360 370 380 390 400VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQGDF YSLLSKLLGE 410 420 430 440 450REDVVHVHKY NPTEKAESES DLVAEIANVV QKKDLGRSDA REGAEHERGN 460 470 480 490 500AILVRDRIHK FHRLVSTLRP PESRVFSLQQ PPPGEGTWEP EHTGDFHMEE 510 520 530 540 550ALDWPGVYLL PGQVSGVALD PKNNLVIFHR GDHVWDGNSF DSKFVYQQIG 560 570 580 590 600LGPIEEDTIL VIDPNNAAVL QSSGKNLFYL PHGLSIDKDG NYWVTDVALH 610 620 630 640 650QVFKLDPNNK EGPVLILGRS MQPGSDQNHF CQPTDVAVDP GTGAIYVSDG 660 670 680 690 700YCNSRIVQFS PSGKFITQWG EESSGSSPLP GQFTVPHSLA LVPLLGQLCV 710 720 730 740 750ADRENGRIQC FKTDTKEFVR EIKHSSFGRN VFAISYIPGL LFAVNGKPHF 760 770 780 790 800GDQEPVQGFV MNFSNGEIID IFKPVRKHFD MPHDIVASED GTVYIGDAHT 810 820 830 840 850NTVWKFTLTE KLEHRSVKKA GIEVQEIKEA EAVVETKMEN KPTSSELQKM 860 870 880 890 900QEKQKLIKEP GSGVPVVLIT TLLVIPVVVL LAIAIFIRWK KSRAFGADSE 910 920 930 940 950HKLETSSGRV LGRFRGKGSG GLNLGNFFAS RKGYSRKGFD RLSTEGSDQE 960 970 KEDDGSESEE EYSAPLPALA PSSS 10 20 30 40 50MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV 60 70 80 90 100PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT 110 120 130 140 150VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG 160 170 180 190 200FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY 210 220 230 240 250LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV 260 270 280 290 300RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH 310 320 330 340 350IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP 360 370 380 390 400VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQGDF YSLLSKLLGE 410 420 430 440 450REDVVHVHKY NPTEKAESES DLVAEIANVV QKKDLGRSDA REGAEHERGN 460 470 480 490 500AILVRDRIHK FHRLVSTLRP PESRVFSLQQ PPPGEGTWEP EHTGDFHMEE 510 520 530 540 550ALDWPGVYLL PGQVSGVALD PKNNLVIFHR GDHVWDGNSF DSKFVYQQIG 560 570 580 590 600LGPIEEDTIL VIDPNNAAVL QSSGKNLFYL PHGLSIDKDG NYWVTDVALH 610 620 630 640 650QVFKLDPNNK EGPVLILGRS MQPGSDQNHF CQPTDVAVDP GTGAIYVSDG 660 670 680 690 700YCNSRIVQFS PSGKFITQWG EESSGSSPLP GQFTVPHSLA LVPLLGQLCV 710 720 730 740 750ADRENGRIQC FKTDTKEFVR EIKHSSFGRN VFAISYIPGL LFAVNGKPHF 760 770 780 790 800GDQEPVQGFV MNFSNGEIID IFKPVRKHFD MPHDIVASED GTVYIGDAHT 810 820 830 840 850NTVWKFTLTE KLEHRSVKKA GIEVQEIKEA EAVVETKMEN KPTSSELQKM 860 870 880 890 900QEKQKLIKEP GSGVPVVLIT TLLVIPVVVL LAIAIFIRWK KSRAFGGKGS 910 920 930 940 950GGLNLGNFFA SRKGYSRKGF DRLSTEGSDQ EKEDDGSESE EEYSAPLPAL APSSS Annotation score:1 out of 5 The annotation score provides a heuristic measure of the annotation content of a Uni Prot KB entry or proteome.

amidating enzyme-26amidating enzyme-22

of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced. This subsection of the 'Subcellular location' section describes the extent of a membrane-spanning region of the protein.Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’. This subsection of the Names and taxonomy section shows the unique identifier assigned by the NCBI to the source organism of the protein.This is known as the ‘taxonomic identifier’ or ‘taxid’. This subsection of the Names and taxonomy section contains the taxonomic hierarchical classification lineage of the source organism.The enzymes, expressed in cells, accomplish this abiological carbon‒nitrogen bond formation via reactive iron-bound carbonyl nitrenes generated from nature-inspired acyl-protected hydroxamate precursors.This transformation is exceptionally efficient (up to 1,020,000 total turnovers) and selective (up to 25:1 regioselectivity and 97%, please refer to compound 2v enantiomeric excess), and can be performed easily on preparative scale.These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting. M37721 m RNA Translation: AAA36414.1S75037 m RNA Translation: AAB32775.1S75038 m RNA Translation: AAB32776.1AB095007 m RNA Translation: BAC22594.1AK290158 m RNA Translation: BAF82847.1BT007419 m RNA Translation: AAP36087.1AC008779 Genomic DNA No translation available. AF035320 m RNA Translation: AAB88190.1NP_000910.2, NM_000919.3 [P19021-5]NP_001170777.1, NM_001177306.1 [P19021-1]NP_001306872.1, NM_001319943.1NP_620121.1, NM_138766.2 [P19021-3]NP_620176.1, NM_138821.2 [P19021-2]NP_620177.1, NM_138822.2 [P19021-4]XP_016864986.1, XM_017009497.1 [P19021-5]XP_016864990.1, XM_017009501.1XP_016864994.1, XM_017009505.1 [P19021-3]ENST00000304400; ENSP00000306100; ENSG00000145730 [P19021-5]ENST00000346918; ENSP00000282992; ENSG00000145730 [P19021-4]ENST00000348126; ENSP00000314638; ENSG00000145730 [P19021-2]ENST00000438793; ENSP00000396493; ENSG00000145730 [P19021-1]ENST00000455264; ENSP00000403461; ENSG00000145730 [P19021-3]M37721 m RNA Translation: AAA36414.1S75037 m RNA Translation: AAB32775.1S75038 m RNA Translation: AAB32776.1AB095007 m RNA Translation: BAC22594.1AK290158 m RNA Translation: BAF82847.1BT007419 m RNA Translation: AAP36087.1AC008779 Genomic DNA No translation available. AF035320 m RNA Translation: AAB88190.1NP_000910.2, NM_000919.3 [P19021-5]NP_001170777.1, NM_001177306.1 [P19021-1]NP_001306872.1, NM_001319943.1NP_620121.1, NM_138766.2 [P19021-3]NP_620176.1, NM_138821.2 [P19021-2]NP_620177.1, NM_138822.2 [P19021-4]XP_016864986.1, XM_017009497.1 [P19021-5]XP_016864990.1, XM_017009501.1XP_016864994.1, XM_017009505.1 [P19021-3]ENST00000304400; ENSP00000306100; ENSG00000145730 [P19021-5]ENST00000346918; ENSP00000282992; ENSG00000145730 [P19021-4]ENST00000348126; ENSP00000314638; ENSG00000145730 [P19021-2]ENST00000438793; ENSP00000396493; ENSG00000145730 [P19021-1]ENST00000455264; ENSP00000403461; ENSG00000145730 [P19021-3]View protein in Inter Pro IPR011042 6-blade_b-propeller_Tol B-like IPR014784 Cu2_ascorb_m Oase-like_CIPR020611 Cu2_ascorb_m Oase_CS-1IPR014783 Cu2_ascorb_m Oase_CS-2IPR000323 Cu2_ascorb_m Oase_NIPR036939 Cu2_ascorb_m Oase_N_sf IPR024548 Cu2_monoox_CIPR001258 NHL_repeat IPR013017 NHL_repeat_subgr IPR000720 PHM/PALIPR008977 PHM/PNGase_F_dom_sf This subsection of the ‘Entry information’ section provides a mnemonic identifier for a Uni Prot KB entry, but it is not a stable identifier.

Each reviewed entry is assigned a unique entry name upon integration into Uni Prot KB/Swiss-Prot. This subsection of the ‘Entry information’ section provides one or more accession number(s).

Additionally, this section gives relevant information on each alternative protein isoform. 10 20 30 40 50MAGRVPSLLV LLVFPSSCLA FRSPLSVFKR FKETTRPFSN ECLGTTRPVV 60 70 80 90 100PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR IPVDEEAFVI DFKPRASMDT 110 120 130 140 150VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA PPTRLPKGVG 160 170 180 190 200FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY 210 220 230 240 250LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV 260 270 280 290 300RNGQWTLIGR QSPQLPQAFY PVGHPVDVSF GDLLAARCVF TGEGRTEATH 310 320 330 340 350IGGTSSDEMC NLYIMYYMEA KHAVSFMTCT QNVAPDMFRT IPPEANIPIP 360 370 380 390 400VKSDMVMMHE HHKETEYKDK IPLLQQPKRE EEEVLDQGDF YSLLSKLLGE 410 420 430 440 450REDVVHVHKY NPTEKAESES DLVAEIANVV QKKDLGRSDA REGAEHERGN 460 470 480 490 500AILVRDRIHK FHRLVSTLRP PESRVFSLQQ PPPGEGTWEP EHTGDFHMEE 510 520 530 540 550ALDWPGVYLL PGQVSGVALD PKNNLVIFHR GDHVWDGNSF DSKFVYQQIG 560 570 580 590 600LGPIEEDTIL VIDPNNAAVL QSSGKNLFYL PHGLSIDKDG NYWVTDVALH 610 620 630 640 650QVFKLDPNNK EGPVLILGRS MQPGSDQNHF CQPTDVAVDP GTGAIYVSDG 660 670 680 690 700YCNSRIVQFS PSGKFITQWG EESSGSSPLP GQFTVPHSLA LVPLLGQLCV 710 720 730 740 750ADRENGRIQC FKTDTKEFVR EIKHSSFGRN VFAISYIPGL LFAVNGKPHF 760 770 780 790 800GDQEPVQGFV MNFSNGEIID IFKPVRKHFD MPHDIVASED GTVYIGDAHT 810 820 830 840 850NTVWKFTLTE KLEHRSVKKA GIEVQEIKEA EAVVETKMEN KPTSSELQKM 860 870 880 890 900QEKQKLIKEP GSGVPVVLIT TLLVIPVVVL LAIAIFIRWK KSRAFGDSEH 910 920 930 940 950KLETSSGRVL GRFRGKGSGG LNLGNFFASR KGYSRKGFDR LSTEGSDQEK 960 970 EDDGSESEEE YSAPLPALAP SSS The checksum is a form of redundancy check that is calculated from the sequence. It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low. This score cannot be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein. Annotation score:1 out of 5 The annotation score provides a heuristic measure of the annotation content of a Uni Prot KB entry or proteome.

However Uni Prot KB may contain entries with identical sequences in case of multiple genes (paralogs). This score cannot be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein. Annotation score:1 out of 5 The annotation score provides a heuristic measure of the annotation content of a Uni Prot KB entry or proteome.

A major challenge in carbon‒hydrogen (C‒H) bond functionalization is to have the catalyst control precisely where a reaction takes place.

In this study, we report engineered cytochrome P450 enzymes that perform unprecedented enantioselective C‒H amidation reactions and control the site selectivity to divergently construct β-, γ-, and δ-lactams, completely overruling the inherent reactivities of the C‒H bonds.

The first step, catalyzed by peptidyl alpha-hydroxylating monoxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate (Pub Med:12699694).